Murakami N, Yoshida A, Ichii S
Endocrinol Jpn. 1979 Feb;26(1):75-80. doi: 10.1507/endocrj1954.26.75.
To examine the binding specificity of steroid hormone-cytoplasmic receptor complexes to nuclei, binding of 3H-dexamethasone (Dex)-liver, 3H-Dex-thymus and 3H-dihydrotestosterone (DHT)-prostate receptor complexes to nuclei from liver, prostate, thymus, spleen and kidney was studied. It was observed that a significant amount of steroid-receptor complexes was bound to any nuclei used in the present study and the extent of the binding of receptor complexes to nuclei from homologous tissues was not always greater than that to nuclei from heterogenous tissues. However, a significant portion of the 3H-Dex-liver and 3H-DHT-prostate receptor complexes was not absorbed by nuclei from kidney, spleem, and thymus, and the unabsorbed complexes were efficiently bound to liver and prostate nuclei. The results obtained indicate that two types of receptor complex with regard to nuclear binding were present in cytosols of liver and prostate; one binds to nuclei from kidney, spleen, thymus, liver and prostate and the other does not bind to nuclei from kidney, spleen and thymus but does bind to nuclei of liver and prostate. The latter type of receptor complex was not observed in the cytosol from the thymus.
为了研究类固醇激素 - 细胞质受体复合物与细胞核的结合特异性，我们研究了³H - 地塞米松（Dex） - 肝脏、³H - Dex - 胸腺和³H - 二氢睾酮（DHT） - 前列腺受体复合物与肝脏、前列腺、胸腺、脾脏和肾脏细胞核的结合情况。结果发现，在本研究中使用的任何细胞核都能结合大量的类固醇 - 受体复合物，并且受体复合物与同源组织细胞核的结合程度并不总是大于与异源组织细胞核的结合程度。然而，³H - Dex - 肝脏和³H - DHT - 前列腺受体复合物的很大一部分不会被肾脏、脾脏和胸腺的细胞核吸收，未被吸收的复合物能有效地结合到肝脏和前列腺细胞核上。所得结果表明，肝脏和前列腺的胞质溶胶中存在两种关于细胞核结合的受体复合物类型；一种能与肾脏、脾脏、胸腺、肝脏和前列腺的细胞核结合，另一种不与肾脏、脾脏和胸腺的细胞核结合，但能与肝脏和前列腺的细胞核结合。在胸腺的胞质溶胶中未观察到后一种类型的受体复合物。
