Maki L R, Galyan E L, Chang-Chien M M, Caldwell D R
Appl Microbiol. 1974 Sep;28(3):456-9. doi: 10.1128/am.28.3.456-459.1974.
Broth cultures of suspensions of Pseudomonas syringae isolated from decaying alder leaves (Alnus tenuifolia) were found to freeze at very warm (-1.8 to -3.8 C) temperatures. The initiation of freezing appears associated with the intact cell and not with extracellular material. Chemical treatments and physical destruction of the cell destroy activity. Bacteria must be in concentrations of approximately 10(6)/ml before freezing at warm temperatures occurs.
从腐烂的桤木叶（细叶桤木）中分离出的丁香假单胞菌悬浮液的肉汤培养物在非常温暖的温度（-1.8至-3.8摄氏度）下会结冰。结冰的起始似乎与完整细胞有关，而不是与细胞外物质有关。化学处理和细胞的物理破坏会破坏活性。在温暖温度下结冰之前，细菌的浓度必须约为10（6）/毫升。
