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1
Properties of two homologous alkaline proteases from Streptomyces rectus.来自直链链霉菌的两种同源碱性蛋白酶的特性
J Bacteriol. 1974 Dec;120(3):1109-15. doi: 10.1128/jb.120.3.1109-1115.1974.
2
The proteolytic enzymes of the K-1 strain of Streptomyces griseus obtained from a commercial preparation (Pronase). I. Purification of four serine endopeptidases.从市售制剂（链霉蛋白酶）中获得的灰色链霉菌K-1菌株的蛋白水解酶。I. 四种丝氨酸内肽酶的纯化。
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本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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Disk electrophoresis of basic proteins and peptides on polyacrylamide gels.碱性蛋白质和肽在聚丙烯酰胺凝胶上的圆盘电泳。
Nature. 1962 Jul 21;195:281-3. doi: 10.1038/195281a0.
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EQUILIBRIUM ULTRACENTRIFUGATION OF DILUTE SOLUTIONS.稀溶液的平衡超速离心法
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Spectroscopic determination of tryptophan and tyrosine in proteins.蛋白质中色氨酸和酪氨酸的光谱测定
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6
Study of the dansylation reaction of amino acids, peptides and proteins.氨基酸、肽和蛋白质的丹磺酰化反应研究。
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7
Thermophilic Streptomyces alkaline proteinase. I. Isolation, crystallization, and physicochemical properties.
J Biol Chem. 1972 Nov 10;247(21):6978-84.
8
The elastase-like enzymes from Streptomyces griseus (pronase). Isolation and partial characterization.来自灰色链霉菌的类弹性蛋白酶（链霉蛋白酶）。分离及部分特性鉴定。
Eur J Biochem. 1971 Mar 1;19(1):90-6. doi: 10.1111/j.1432-1033.1971.tb01291.x.
9
Serine-containing active center of alkaline proteinase of Aspergillus flavus.黄曲霉碱性蛋白酶含丝氨酸的活性中心。
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来自直链链霉菌的两种同源碱性蛋白酶的特性

Properties of two homologous alkaline proteases from Streptomyces rectus.

作者信息

Borgia P, Campbell L L

出版信息

J Bacteriol. 1974 Dec;120(3):1109-15. doi: 10.1128/jb.120.3.1109-1115.1974.

DOI:10.1128/jb.120.3.1109-1115.1974
PMID:4373436
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC245889/
Abstract

Some physicochemical properties of two thermostable proteases from Streptomyces rectus are described. The enzymes were judged to be identical with respect to molecular weight, inactivation with serine protease inhibitors, and in primary structure by peptide analysis. Amino acid analysis indicated the enzymes had identical compositions except for their amide content. The molecular weights of the enzymes were judged to be 28,000 by sedimentation equilibrium, 26,200 by sedimentation diffusion, and 29,100 from amino acid analysis. Titration of the proteases with diisopropylfluorophosphate and phenylmethane sulfonylfuoride indicate equivalent weights of 28,500 and 32,800 g, respectively, for the proteins. The pentapeptide around the serine residue reacting with diisopropylfluorophosphate was isolated and had the composition: Asx(1), Gly(1), Thr(1), Ser(1), Met(1).

摘要

本文描述了直链链霉菌两种热稳定蛋白酶的一些物理化学性质。通过肽分析判断，这两种酶在分子量、对丝氨酸蛋白酶抑制剂的失活作用以及一级结构方面均相同。氨基酸分析表明，除酰胺含量外，这两种酶的组成相同。通过沉降平衡法测得酶的分子量为28,000，沉降扩散法测得为26,200，氨基酸分析法测得为29,100。用二异丙基氟磷酸酯和苯甲基磺酰氟滴定蛋白酶表明，蛋白质的当量分别为28,500和32,800 g。分离出了与二异丙基氟磷酸酯反应的丝氨酸残基周围的五肽，其组成为：天冬氨酸（1）、甘氨酸（1）、苏氨酸（1）、丝氨酸（1）、甲硫氨酸（1）。