Koch A S, Eremenko T, Benedetto A, Volpe P
Intervirology. 1974;4(4):221-5. doi: 10.1159/000149966.
It has been shown that the amount of viral RNA synthesized in synchronized HeLa cells 0-2 h after infection does not notably vary during the mitotic cycle. However, the amount of viral RNA synthesized 2-4.5 h after infection exhibited a sharp maximum in S-phase cells. Guanidine inhibited the incorporation of 3-H-uridine into the viral RNA synthesized 2-4.5 h after infection only in cells in the S-phase. It had no demonstrable effect on viral RNA synthesis 0-2 h after infection in cells in any phase of the mitotic cycle. Thus, additional indirect evidence has been presented in favor of the hypothesis that two different replicases or two different conformations of one enzyme are involved in poliovirus RNA replication. The one functioning 0-2 h after infection is cycle-independent and guanidine-sensitive, whereas the other functions exclusively in phase-S cells 2-4.5 h after infection and is inhibited by guanidine.
已表明，在同步化的HeLa细胞中，感染后0至2小时合成的病毒RNA量在有丝分裂周期中没有显著变化。然而，感染后2至4.5小时合成的病毒RNA量在S期细胞中呈现出急剧的最大值。胍仅在S期细胞中抑制感染后2至4.5小时合成的病毒RNA中3-H-尿苷的掺入。它对有丝分裂周期任何阶段的细胞在感染后0至2小时的病毒RNA合成没有明显影响。因此，已经提出了额外的间接证据支持这一假设，即脊髓灰质炎病毒RNA复制涉及两种不同的复制酶或一种酶的两种不同构象。一种在感染后0至2小时起作用，与周期无关且对胍敏感，而另一种仅在感染后2至4.5小时的S期细胞中起作用，并被胍抑制。
