Milton J D
Immunology. 1979 Mar;36(3):533-8.
Carbon blockade of mice did not affect cytotoxic antibody production after immunization with allogeneic cells, nor did it affect the immunosuppressive activity of alloantibody. Though it inhibited the alloantibody-mediated increase in hepatic localization of allogeneic lymphoid cells it had no effect on the normal hepatic localization of allogeneic lymphoid cells. Alloantibody administered before allogeneic cells or 6 h after was equally immunosuppressive, but anti-lymphocyte antibody only had potent immunosuppressive activity when given just before the antigenic cells. These results suggest than an afferent mechanism of suppression of the humoral antibody response can be exerted by anti-lymphocyte serum and that alloantibody may also act by this method when given before the antigen. As alloantibody is also highly potent when given after the antigen when an afferent mechanism is unlikely to occur, studies on the specificity of immunosuppression, particularly the likely requirement for antibody to I region determinants, would best be conducted by giving the antibody after the antigen, thereby avoiding any possible afferent inhibitory activity.
对小鼠进行碳阻断并不影响用同种异体细胞免疫后的细胞毒性抗体产生，也不影响同种异体抗体的免疫抑制活性。虽然它抑制了同种异体抗体介导的同种异体淋巴细胞肝定位增加，但对同种异体淋巴细胞的正常肝定位没有影响。在同种异体细胞之前或之后6小时给予的同种异体抗体具有同等的免疫抑制作用，但抗淋巴细胞抗体只有在抗原细胞之前给予时才具有强大的免疫抑制活性。这些结果表明，抗淋巴细胞血清可通过传入机制抑制体液抗体反应，并且同种异体抗体在抗原之前给予时也可能通过这种方式起作用。由于同种异体抗体在抗原之后给予时（此时不太可能发生传入机制）也具有高效力，因此关于免疫抑制特异性的研究，特别是对I区决定簇抗体的可能需求，最好在抗原之后给予抗体来进行，从而避免任何可能的传入抑制活性。
