Amarger N, Alexander M
J Bacteriol. 1968 May;95(5):1651-7. doi: 10.1128/jb.95.5.1651-1657.1968.
Nitrite was formed from hydroxylamine and several oximes by intact cells and extracts of Pseudomonas aeruginosa. The activity was induced by the presence of oximes in the culture medium. Nitroalkanes were not intermediates in the conversion of acetaldoxime, acetone oxime, or butanone oxime to nitrite, since nitromethane inhibited the formation of nitrite from the nitro compounds but not from the corresponding oximes. The oxime apparently functions as a constant source of hydroxylamine during growth of the bacterium. Hydroxylamine at low concentration was converted stoichiometrically to nitrite by extracts of the bacterium; high concentrations were inhibitory. Nicotinamide adenine dinucleotide phosphate, oxygen, and other unidentified cofactors were necessary for the reaction. Actively nitrifying extracts possessed no hydroxylamine-cytochrome c reductase activity. Hyponitrite, nitrous oxide, and nitric oxide were not metabolized.
铜绿假单胞菌的完整细胞和提取物可将羟胺和几种肟转化为亚硝酸盐。培养基中肟的存在可诱导该活性。硝基烷不是乙醛肟、丙酮肟或丁酮肟转化为亚硝酸盐的中间体，因为硝基甲烷抑制了硝基化合物生成亚硝酸盐，但不抑制相应肟生成亚硝酸盐。在细菌生长过程中，肟显然作为羟胺的恒定来源发挥作用。低浓度的羟胺可被细菌提取物化学计量地转化为亚硝酸盐；高浓度则具有抑制作用。烟酰胺腺嘌呤二核苷酸磷酸、氧气和其他未鉴定的辅助因子是该反应所必需的。具有活性的硝化提取物不具有羟胺 - 细胞色素c还原酶活性。连二次硝酸、一氧化二氮和一氧化氮都不能被代谢。
