Williams J F, Rienits K G, Clark M G
Biochem J. 1971 Aug;123(5):915-22. doi: 10.1042/bj1230915.
A surgical procedure for the isolation of the liver from the systemic circulation of the anaesthetized rabbit is described. The technique allowed the metabolism in situ of intraportally infused substrates to be followed for periods up to 5min, free from the contaminating influences of metabolism by other body tissues. Details of the procedures necessary to achieve the uniform infusion, homogeneous distribution and containment of (14)C-labelled glucose substrates in the liver by haemostasis are described. Changes in pO(2), pCO(2), pH and the concentrations of NADP(+), NADPH and glucose during each minute interval of the total 5min period of metabolism are given. Reactant ratios of the lactate dehydrogenase system and the adenine nucleotide system have been calculated from the concentrations of the pertinent metabolites for the same period of metabolism. Glucose production by rabbit liver in situ proceeded at the rate of 1.08mumol/min per g wet wt. of liver during the 5min metabolic interval. The presence of the oxidative reactions of the pentose phosphate pathway of glucose metabolism was inferred from the quotient oxidation of [1-(14)C]glucose/oxidation of [6-(14)C]glucose=1.8.
本文描述了一种用于在麻醉兔体内将肝脏与体循环隔离的外科手术方法。该技术能够在长达5分钟的时间内追踪经门静脉注入的底物在肝脏中的原位代谢情况，且不受其他身体组织代谢的干扰影响。文中详细说明了通过止血实现（14）C标记的葡萄糖底物在肝脏中均匀输注、均匀分布和留存所需的手术步骤。给出了在总共5分钟的代谢期内，每分钟间隔的pO₂、pCO₂、pH以及NADP⁺、NADPH和葡萄糖浓度的变化情况。根据相同代谢期内相关代谢物的浓度，计算了乳酸脱氢酶系统和腺嘌呤核苷酸系统的反应物比率。在5分钟的代谢间隔内，兔肝脏原位葡萄糖生成速率为每克湿重肝脏1.08μmol/分钟。由[1-（14）C]葡萄糖氧化/[6-（14）C]葡萄糖氧化的商数为1.8，推断出葡萄糖代谢的磷酸戊糖途径存在氧化反应。
