肝癌细胞杂交体中分化功能的表达：染色体丢失后酪氨酸转氨酶诱导性的重现。
Expression of differentiated functions in hepatoma cell hybrids: reappearance of tyrosine aminotransferase inducibility after the loss of chromosomes.
作者信息
Weiss M C, Chaplain M
出版信息
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3026-30. doi: 10.1073/pnas.68.12.3026.
Abstract
HYBRIDS FROM A CROSS OF RAT HEPATOMA CELLS WITH DIPLOID EPITHELIAL CELLS FROM RAT LIVER HAVE BEEN STUDIED WITH RESPECT TO KARYOTYPE AND EXPRESSION OF TWO FUNCTIONS LIMITED TO THE HEPATOMA PARENT
high level of the enzyme tyrosine aminotransferase (EC 2.6.1.5; L-tyrosine:2-oxoglutarate aminotransferase) and its inducibility with steroid hormones. The hybrids that contain the complete chromosomal complements from both parents show low enzyme activity and no inducibility. One hybrid clone, and all of its derivatives, which have lost 30-40% of the chromosomes initially present, show enzyme inducibility. Induction of tyrosine aminotransferase in the hepatoma and hybrid cells responds similarly to inhibition by cycloheximide and actinomycin D, and to steroid concentration. The enzymes from induced and noninduced hepatoma cells and from induced hybrid cells are similar in heat sensitivity and intracellular distribution; those from noninduced hybrid and diploid rat epithelial cells are different.
摘要
对大鼠肝癌细胞与大鼠肝脏二倍体上皮细胞杂交产生的杂种细胞进行了研究，涉及核型以及仅限于肝癌亲本的两种功能的表达：高水平的酪氨酸转氨酶（EC 2.6.1.5；L-酪氨酸：2-氧代戊二酸转氨酶）及其对类固醇激素的诱导性。含有双亲完整染色体组的杂种细胞显示出低酶活性且无诱导性。一个杂种克隆及其所有衍生物，它们最初存在的染色体已丢失30 - 40%，显示出酶诱导性。肝癌细胞和杂种细胞中酪氨酸转氨酶的诱导对环己酰亚胺和放线菌素D的抑制以及对类固醇浓度的反应相似。来自诱导和未诱导的肝癌细胞以及诱导杂种细胞的酶在热敏感性和细胞内分布方面相似；来自未诱导杂种细胞和二倍体大鼠上皮细胞的酶则不同。
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