Roubein I F, Embree L J
Res Commun Chem Pathol Pharmacol. 1979 Jan;23(1):143-53.
Male Sprague-Dawley rats (200-300 g) were sacrificed by cervical dislocation and left either at room temperature (21-23 degrees C) for periods of 1, 2, and 4 hours or in the refrigerator (4-5 degrees C) for periods of 1, 2, 4, 8, 16, and 24 hours. The brains were left in the intact animals in order to stimulate human autopsy materials. At the end of each period, the rat was decapitated and the brain removed and immediately dissected into the following regions: cerebral cortex, striatum, midbrain, pons and medulla, and cerebellum. The levels of norepinephrine (NE) and dopamine (DA) in brain regions were determined fluorometrically. In animals kept at room temperature the level of NE decreased gradually with time reaching 61%, 76%, 68%, and 68% of control in the cerebral cortex, midbrain, pons and medulla, and cerebellum at 4 hours post mortem respectively. Striatal DA reached 61% of control at 2 hours and remained the same at 4 hours post mortem. The NE level declined at a slower rate in brain regions obtained from refrigerated animals, reaching 65%, 81%, 77%, and 77% of control in cerebral cortex, midbrain, pons and medulla, and cerebellum at 24 hours post mortem. Striatal DA reached 54% of control at 16 hours and showed no further decline at 54 hours post mortem. There was a significant post mortem decrease in NE and DA levels in all brain regions examined, however, the rate of this decline varied from region to region. The tissues obtained from refrigerated animals showed statistically less of a decline in amine levels.
将体重200 - 300克的雄性斯普拉格 - 道利大鼠通过颈椎脱臼法处死，然后分别在室温（21 - 23摄氏度）下放置1小时、2小时和4小时，或在冰箱（4 - 5摄氏度）中放置1小时、2小时、4小时、8小时、16小时和24小时。为了模拟人类尸检材料，大脑保留在完整的动物体内。在每个时间段结束时，将大鼠断头，取出大脑并立即解剖成以下区域：大脑皮层、纹状体、中脑、脑桥和延髓以及小脑。采用荧光法测定脑区中去甲肾上腺素（NE）和多巴胺（DA）的水平。在室温下保存的动物中，NE水平随时间逐渐下降，在死后4小时时，大脑皮层、中脑、脑桥和延髓以及小脑中的NE水平分别降至对照值的61%、76%、68%和68%。纹状体DA在死后2小时降至对照值的61%，并在死后4小时保持不变。从冷藏动物获得的脑区中，NE水平下降速度较慢，在死后24小时时，大脑皮层、中脑、脑桥和延髓以及小脑中的NE水平分别降至对照值的65%、81%、77%和77%。纹状体DA在死后16小时降至对照值的54%，并在死后54小时没有进一步下降。在所检查的所有脑区中，NE和DA水平在死后均显著下降，然而，这种下降速度因区域而异。从冷藏动物获得的组织在胺水平下降方面在统计学上较少。
