Greve T, Lehn-Jensen H
Acta Vet Scand. 1979;20(1):135-44. doi: 10.1186/BF03546636.
Previous attempts to transfer embryos non-surgically to heifers have been rather discouraging. The present experiment describes a simple non-surgical technique where the 6½–7½ -day old embryo is placed in a 0.25 ml ministraw, fitted into a common insemination gun and transferred cervically to synchronized lactating dairy cows. Thirty-two viable embryos were transferred to the middle part of the uterine horn ipsilateral to the ovary containing the corpus luteum. It was easy to pass the cervix, and most embryos could be deposited in the horn within 1 or 2 min. Eighteen animals were diagnosed pregnant (56% ). It is conceivable that the easy atraumatic transfers and good management (feeding, oestrus control) of the recipients contributed to the high pregnancy rate.
以往非手术方式向小母牛移植胚胎的尝试结果颇为令人沮丧。本实验描述了一种简单的非手术技术,即将6.5至7.5日龄的胚胎置于0.25毫升的细管中,装入普通授精枪,经子宫颈移植到同期发情的泌乳奶牛体内。将32个存活胚胎移植到卵巢有黄体一侧子宫角的中部。通过子宫颈很容易,大多数胚胎能在1或2分钟内被植入子宫角。18头动物被诊断怀孕(56%)。可以想象,受体易于操作且无创伤的移植以及良好的管理(饲养、发情控制)促成了高怀孕率。