Devous M D, Jones C E
Cardiology. 1979;64(3):149-61. doi: 10.1159/000170612.
In 16 dogs inosine was infused at 0.5 mmol/min i.v. for 5 min beginning 15 min after coronary occlusion. Tracer microspheres were used to estimate flow in subepicardium and subendocardium of nonischemic, central ischemic, and borderline ischemic muscle. Estimates of flow before occlusion, 5 min after occlusion, during inosine infusion, 30 min after infusion and 60 min after infusion were obtained. Coronary occlusion reduced flow in the central ischemic regions by 75-95%. The reduction in flow was greatest in subendocardium. In the borderline regions subendocardial flow was reduced by 30% while subepicardial flow was unaffected. The major effects of inosine were seen in nonischemic and borderline ischemic regions. Flow in borderline subendocardium returned to its pre-occlusion value, and flow in nonischemic myocardium increased by approximately 60-80%. However, only in the ischemic regions was the increase in flow sustained for the entire 60 min. In 20 dogs infarct size was determined using nitro blue tetrazolium stain. In 10 controls infarct size was 20.1%, while in 10 inosine-treated dogs infarct size was 15.2% of left ventricular weight (p less than 0.01). Thus, following coronary occlusion inosine infusion was associated with an increase in perfusion of ischemic myocardium and a reduction in infarct size.
在16只犬中，冠状动脉闭塞15分钟后开始以0.5 mmol/分钟的速度静脉输注肌苷，持续5分钟。使用示踪微球估计非缺血、中心缺血和边缘缺血心肌的心外膜下和心内膜下血流。获得了闭塞前、闭塞后5分钟、肌苷输注期间、输注后30分钟和输注后60分钟的血流估计值。冠状动脉闭塞使中心缺血区域的血流减少了75 - 95%。血流减少在心内膜下最为显著。在边缘区域，心内膜下血流减少了30%，而心外膜下血流未受影响。肌苷的主要作用见于非缺血和边缘缺血区域。边缘心内膜下血流恢复到闭塞前的值，非缺血心肌的血流增加了约60 - 80%。然而，只有在缺血区域，血流增加才持续了整个60分钟。在20只犬中，使用硝基蓝四氮唑染色法测定梗死面积。在10只对照犬中，梗死面积为左心室重量的20.1%，而在10只接受肌苷治疗的犬中，梗死面积为左心室重量的15.2%（p小于0.01）。因此，冠状动脉闭塞后输注肌苷与缺血心肌灌注增加和梗死面积减小有关。
