Astvatsatur'ian A T, Staviskiĭ I M, Gubareva A E, Alimova E K
Vopr Med Khim. 1979 May-Jun;25(3):314-8.
Ability of epithelium of rat small intestine mucose to synthetize arachidonic acid was studied in vivo and in vitro. Linoleic acid and 2-14C-acetate were used as precursors. The fatty acids were fractionated using thin-layer chromatography on silica gel impregnated with AgNO3. The label was incorporated into all the fractions but the highest radioactivity was found in the fraction of arachidonic acid; this suggests that the enzyme systems, carrying out the synthesis of polyenic fatty acids, occur in the mucosa of small intestine.
在体内和体外研究了大鼠小肠黏膜上皮合成花生四烯酸的能力。以亚油酸和2-¹⁴C-醋酸盐作为前体。脂肪酸通过在浸渍有硝酸银的硅胶上进行薄层色谱分离。标记物掺入了所有组分中,但在花生四烯酸组分中发现了最高的放射性;这表明进行多烯脂肪酸合成的酶系统存在于小肠黏膜中。