Chen W P, Anderson A W, Han Y W
Appl Environ Microbiol. 1979 Apr;37(4):785-7. doi: 10.1128/aem.37.4.785-787.1979.
Cationic detergent (cetyltrimethylammonium bromide or cetylpyridinium chloride) treatment extracted almost the same amount of glucose isomerase from cells of Streptomyces flavogriseus as mechanical disruption (sonic oscillation or abrasive grinding). The specific activity of the enzyme extracted with cationic detergents was approximately 20% higher than that liberated by mechanical disruption.
阳离子洗涤剂(十六烷基三甲基溴化铵或十六烷基氯化吡啶)处理从黄色链霉菌细胞中提取的葡萄糖异构酶量与机械破碎(超声振荡或研磨)提取的量几乎相同。用阳离子洗涤剂提取的酶的比活性比通过机械破碎释放的酶的比活性高约20%。