Lakshmi S, Balasubramanian A S
Biochim Biophys Acta. 1979 Mar 16;567(1):184-95. doi: 10.1016/0005-2744(79)90185-2.
Arylsulfatase C (aryl-sulfate sulfohydrolase, EC 3.1.6.1) from sheep brain acetone powder was solubilized with the chaotropic agent, KSCN. Anti-chaotropes such as (NH4)2SO4 or sodium citrate significantly enhanced the activity of the solubilized enzyme indicating that hydrophobicity was an important factor influencing the enzyme activity. Dialysis or gel filtration of the solubilized enzyme resulted in a marked loss of activity. 3a dialyzable activator could reconstitute the activity in the presence of the antichaotropes. The activator was purified partially and preliminary studies indicated it to be a low molecular weight peptide. Arylsulfatase C and estrone sulfatase activities were compared in the solubilized enzyme. Estrone sulfatase activity was also increased in the presence of antichaotropes at lower concentration in comparison to arylsulfatase C. It however did not show a requirement for the dialyzable activator. Kinetic studies showed that elevation of enzyme activity by the antichaotropes and activator in the case of arylsulfatase C and by antichaotropes in the case of estrone sulfatase was due to an increase in V with a decrease in Km.
用离液剂硫氰酸钾（KSCN）溶解来自羊脑丙酮粉的芳基硫酸酯酶C（芳基硫酸硫酸水解酶，EC 3.1.6.1）。诸如硫酸铵或柠檬酸钠等抗离液剂显著增强了溶解酶的活性，表明疏水性是影响酶活性的一个重要因素。对溶解酶进行透析或凝胶过滤会导致活性显著丧失。一种可透析的激活剂能在抗离液剂存在的情况下恢复活性。该激活剂得到了部分纯化，初步研究表明它是一种低分子量肽。对溶解酶中的芳基硫酸酯酶C和雌酮硫酸酯酶活性进行了比较。与芳基硫酸酯酶C相比，在较低浓度的抗离液剂存在下，雌酮硫酸酯酶活性也有所增加。然而，它并不需要可透析的激活剂。动力学研究表明，对于芳基硫酸酯酶C，抗离液剂和激活剂提高酶活性，而对于雌酮硫酸酯酶，抗离液剂提高酶活性，这是由于V增加而Km降低所致。
