Leppard G G, Colvin J R
J Cell Biol. 1972 Jun;53(3):695-703. doi: 10.1083/jcb.53.3.695.
The components of higher-plant cell walls which become electron-opaque after staining with ruthenium-osmium were studied by electron microscopy. A fibrillar material which absorbs this stain is a major wall constituent in the root epidermal cells of carrot and morning glory. In both form and size, these fibrils resemble those found on the surface of suspension-cultured cells of the same species Some cells of woody species show an irregular distribution of electron-opaque material in the cell wall matrix and middle lamella. This material, which has an amorphous appearance with many electron stains, is shown by ruthenium-osmium staining to be an aggregate of discrete granules, 150-220 A in diameter. These observations are not consistent with the concept of the cell wall matrix and middle lamella as an amorphous, uniform gel
用钌锇染色后变得电子不透明的高等植物细胞壁成分通过电子显微镜进行了研究。一种吸收这种染色剂的纤维状物质是胡萝卜和牵牛花根表皮细胞中的主要细胞壁成分。在形态和大小上，这些纤维与在相同物种的悬浮培养细胞表面发现的纤维相似。一些木本物种的细胞在细胞壁基质和中胶层中显示出电子不透明物质的不规则分布。这种物质在许多电子染色下呈无定形外观，经钌锇染色显示为直径150 - 220埃的离散颗粒聚集体。这些观察结果与细胞壁基质和中胶层是无定形、均匀凝胶的概念不一致。
