纤细短膜虫在存在羰基氰化物间氯苯腙的情况下生长时，伴随生理适应过程中的代谢变化。

Metabolic changes in Crithidia fasciculata accompanying physiological adaptation to growth in the presence of carbonyl cyanide m-chlorophenylhydrazone.

Kutzman R S, Roberts J F

Department of Zoology, North Carolina State University, Raleigh 27650.

Comp Biochem Physiol B. 1979;62(4):449-53. doi: 10.1016/0305-0491(79)90116-0.

Abstract

Crithidia fasciculata adapted to growth in the presence of 10(-5) M carbonyl cyanide m-chlorophenylhydrazone (CCCP), an uncoupler of oxidative phosphorylation, maintained adenosine phosphate pools and an adenylate energy charge comparable to those of control cells. 2. CCCP-adapted cells in the presence of the uncoupler respire endogenous substrate at a greater rate than control cells and this effect of CCCP appears readily reversible. 3. CCCP-treated, adapted cells, supporting high endogenous respiration rates, were not responsive to added substrates which significantly stimulated the oxygen utilization of normal C. fasciculata. 4. CCCP-adapted cells, provided with [U-14C]-labeled proline, utilize this substrate at 67% the rate of control cells, but divide the isotopic label between CO2 and protein in a ratio identical to that of normal cells. 5. The transport of alanine and proline by adapted C. fasciculata was severely impaired, while the transport of tyrosine and leucine was unaffected.

摘要

适应在10⁻⁵ M羰基氰m -氯苯腙（CCCP，一种氧化磷酸化解偶联剂）存在下生长的束状克氏锥虫，其磷酸腺苷池和腺苷酸能荷与对照细胞相当。2. 在解偶联剂存在下，适应CCCP的细胞比对照细胞以更高的速率呼吸内源性底物，并且CCCP的这种作用似乎很容易逆转。3. 经CCCP处理并适应的细胞，支持高内源性呼吸速率，对添加的底物无反应，而这些底物能显著刺激正常束状克氏锥虫的氧利用。4. 用[U - ¹⁴C]标记的脯氨酸处理适应CCCP的细胞，其利用该底物的速率为对照细胞的67%，但在二氧化碳和蛋白质之间分配同位素标记的比例与正常细胞相同。5. 适应的束状克氏锥虫对丙氨酸和脯氨酸的转运严重受损，而对酪氨酸和亮氨酸的转运未受影响。