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携带质粒的大肠杆菌菌株中的汞抗性

Mercury resistance in a plasmid-bearing strain of Escherichia coli.

作者信息

Summers A O, Silver S

出版信息

J Bacteriol. 1972 Dec;112(3):1228-36. doi: 10.1128/jb.112.3.1228-1236.1972.

Abstract

A strain of Escherichia coli carrying genes determining mercury resistance on a naturally occurring resistance transfer factor (RTF) converts 95% of 10(-5)m Hg(2+) (chloride) to metallic mercury at a rate of 4 to 5 nmoles of Hg(2+) per min per 10(8) cells. The metallic mercury is rapidly eliminated from the culture medium as mercury vapor. The volatilizing activity has a temperature dependence and heat sensitivity characteristic of enzymatic catalysis and is inducible by mercuric chloride. Ag(+) and Au(3+) are markedly inhibitory of mercury volatilization.

摘要

一株携带决定汞抗性基因的大肠杆菌,该基因位于天然存在的抗性转移因子(RTF)上,能将10⁻⁵m Hg²⁺(氯化物)的95%转化为金属汞,转化速率为每10⁸个细胞每分钟4至5纳摩尔Hg²⁺。金属汞以汞蒸气的形式迅速从培养基中消除。挥发活性具有酶催化的温度依赖性和热敏感性特征,并且可被氯化汞诱导。Ag⁺和Au³⁺对汞挥发有明显抑制作用。

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