Dall'Acqua F, Vedaldi D, Bordin F, Rodighiero G
J Invest Dermatol. 1979 Aug;73(2):191-7. doi: 10.1111/1523-1747.ep12581681.
Some aspects of the interactions between DNA and 8-methoxypsoralen (8-MOP) in its ground state (complex formation) or in its excited state (photobinding) have been investigated. 8-MOP shows a low affinity towards DNA in the complex formation; this fact minimizes the possible biological consequences deriving from this interaction, when it occurs in vivo. In covalent photobinding to DNA, 8-MOP forms mainly monofunctional adducts, and to a lesser extent bifunctional adducts, showing a behavior similar to that of other linearly condensed furocoumarins (psoralens); the ratio between mono- and bifunctional adducts was found to be 9:1. The covalent photobinding to DNA does not occur at random along the macromolecule, but preferentially at the level of specific receptor sites. The regions having an alternate sequence of A-T seem to be the best receptor sites for the formation of monoadducts while the regions containing an alternate sequence of A-T and C-G appeared to be the preferential sites for the cross-linkage formation.
已对处于基态（形成复合物）或激发态（光结合）的DNA与8-甲氧基补骨脂素（8-MOP）之间相互作用的某些方面进行了研究。8-MOP在复合物形成过程中对DNA表现出低亲和力；当这种相互作用在体内发生时，这一事实将源于该相互作用的可能生物学后果降至最低。在与DNA的共价光结合中，8-MOP主要形成单功能加合物，较少程度上形成双功能加合物，表现出与其他线性稠合呋喃香豆素（补骨脂素）类似的行为；发现单功能与双功能加合物的比例为9:1。与DNA的共价光结合并非沿着大分子随机发生，而是优先发生在特定受体位点水平。具有A-T交替序列的区域似乎是形成单加合物的最佳受体位点，而含有A-T和C-G交替序列的区域似乎是形成交联的优先位点。
