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锌离子对细菌接合作用的影响:抑制配对形成。

Effect of Zn2+ on bacterial conjugation: inhibition of mating pair formation.

作者信息

Ou J T, Anderson T F

出版信息

J Bacteriol. 1972 Jul;111(1):177-85. doi: 10.1128/jb.111.1.177-185.1972.

Abstract

Zn(2+) at 10(-3)m has been found to block the formation of mating pairs between Hfr and F(-) strains of Escherichia coli as observed both by light microscopy and by Coulter counter measurements. Kinetic studies show that Zn(2+) reduces the fertility of the male and that its effect disappears within 2 min after Zn(2+) has been removed from the medium. Short treatments of female cells with Zn(2+) have no detectable effect on their ability to form mating pairs. Later steps in the mating process such as mobilization of the male chromosome, transfer of the chromosome to the female, or its integration into the female chromosome seem not to be affected by 10(-3)m Zn(2+).

摘要

已发现,10⁻³m的锌离子(Zn²⁺)可阻止大肠杆菌Hfr和F⁻菌株之间配对的形成,这一点通过光学显微镜和库尔特计数器测量均已观察到。动力学研究表明,锌离子会降低雄性菌株的育性,并且在从培养基中去除锌离子后2分钟内其作用就会消失。用锌离子对雌性细胞进行短时间处理,对其形成配对的能力没有可检测到的影响。交配过程中的后续步骤,如雄性染色体的动员、染色体向雌性的转移或其整合到雌性染色体中,似乎不受10⁻³m锌离子的影响。

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