Doolittle W F
J Bacteriol. 1972 Aug;111(2):316-24. doi: 10.1128/jb.111.2.316-324.1972.
Methods are described for preparation of pulse-labeled ribonucleic acid (RNA) from the blue-green alga Anacystis nidulans. Synthesis of labeled RNA was found to be in part dependent on concurrent photosynthesis and was inhibited by the antibiotic streptolydigin. Mature 23S ribosomal RNA (rRNA) appeared before mature 16S rRNA. Formation of either molecule was inhibited by chloramphenicol, and RNA species of lesser mobility accumulated. These species may be precursors of the mature forms. Maturation of 16S rRNA was also inhibited by streptolydigin. (The effect of this antibiotic on 23S rRNA maturation was not examined). In many respects, ribosomal RNA synthesis and maturation in this blue-green alga appear to follow the pattern already established for bacteria.
本文描述了从蓝藻集胞藻中制备脉冲标记核糖核酸(RNA)的方法。发现标记RNA的合成部分依赖于同时进行的光合作用,并受到抗生素链霉溶菌素的抑制。成熟的23S核糖体RNA(rRNA)在成熟的16S rRNA之前出现。氯霉素抑制了这两种分子的形成,并且积累了迁移率较低的RNA种类。这些种类可能是成熟形式的前体。链霉溶菌素也抑制了16S rRNA的成熟。(未检测这种抗生素对23S rRNA成熟的影响)。在许多方面,这种蓝藻中的核糖体RNA合成和成熟似乎遵循已为细菌确立的模式。