Kamiński E, Libbey L M, Stawicki S, Wasowicz E
Appl Microbiol. 1972 Nov;24(5):721-6. doi: 10.1128/am.24.5.721-726.1972.
A culture of Aspergillus flavus grown on moistened wheat meal was homogenized with a blendor, and the resulting slurry was vacuum-distilled at 5 mm of Hg and 35 C. The aqueous distillate was collected in traps cooled to -10 to -80 C. The culture volatiles were extracted from the distillate with CH(2)Cl(2), and, after removal of the bulk of the solvent, the concentrated volatiles were examined by packed-column gas chromatography. Nineteen peaks were observed, and coupled gas chromatography-mass spectrometry was employed to identify the larger components. The compounds identified were: 3-methyl-butanol, 3-octanone, 3-octanol, 1-octen-3-ol, 1-octanol, and cis-2-octen-1-ol. The two octenols were the predominant compounds, and sufficient sample was trapped from the gas chromatograph for infrared analyses; this confirmed the mass spectral identifications and permitted the assignment of the cis designation to 2-octen-1-ol. Both oct-1-en-3-ol and cis-2-octen-1-ol are thought to be responsible for the characteristic musty-fungal odor of certain fungi; the latter compound may be a useful chemical index of fungal growth.
将在湿润小麦粉上培养的黄曲霉培养物用搅拌机匀浆，所得浆液在5毫米汞柱和35℃下进行减压蒸馏。含水馏出物收集在冷却至-10至-80℃的捕集器中。用二氯甲烷从馏出物中萃取培养物挥发物，在除去大部分溶剂后，用填充柱气相色谱法检测浓缩的挥发物。观察到19个峰，并采用气相色谱-质谱联用技术鉴定较大的组分。鉴定出的化合物有：3-甲基-丁醇、3-辛酮、3-辛醇、1-辛烯-3-醇、1-辛醇和顺式-2-辛烯-1-醇。两种辛烯醇是主要化合物，从气相色谱仪中捕集了足够的样品用于红外分析；这证实了质谱鉴定结果，并确定了2-辛烯-1-醇的顺式构型。1-辛烯-3-醇和顺式-2-辛烯-1-醇都被认为是某些真菌特有的霉味的来源；后一种化合物可能是真菌生长的有用化学指标。
