胎儿和成年豚鼠肾上腺皮质内层细胞中的过氧化物酶体。
Peroxisomes in inner adrenocortical cells of fetal and adult guinea pigs.
作者信息
Black V H, Bogart B I
出版信息
J Cell Biol. 1973 May;57(2):345-58. doi: 10.1083/jcb.57.2.345.
Abstract
Abundant membrane-bounded granules, 0.1-0.45 microm in diameter, occur among the elements of the smooth-surfaced endoplasmic reticulum in zona fasciculata and zona reticularis adrenocortical cells of guinea pigs. Acid phosphatase cannot be cytochemically demonstrated in them, and they are therefore distinct from lysosomes. Incubation in medium containing 3,3'-diaminobenzidine results in dense staining of the granules, identifying them as peroxisomes. These small peroxisomes increase in number as fetal adrenocortical cells differentiate, and they appear to arise from dilated regions of endoplasmic reticulum. They maintain interconnections with the smooth endoplasmic reticulum and with one another.
摘要
在豚鼠束状带和网状带肾上腺皮质细胞的滑面内质网成分中，存在大量直径为0.1 - 0.45微米的膜结合颗粒。酸性磷酸酶在其中无法通过细胞化学方法显示，因此它们与溶酶体不同。在含有3,3'-二氨基联苯胺的培养基中孵育会导致颗粒密集染色，将它们鉴定为过氧化物酶体。随着胎儿肾上腺皮质细胞的分化，这些小的过氧化物酶体数量增加，并且它们似乎起源于内质网的扩张区域。它们与滑面内质网以及彼此之间保持相互连接。
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