两类发育中大鼠脑细胞核中非组蛋白染色体蛋白的代谢稳定性
Metabolic stability of nonhistone chromosomal proteins in two different classes of developing rat brain nuclei.
作者信息
Stambolova M A, Angelova A A, Tsanev R G
出版信息
Cell Differ. 1979 Jun;8(3):195-202. doi: 10.1016/0045-6039(79)90046-0.
The metabolic behaviour of NHCP was studied in rat brain nuclei from fully differetiated cells and in nuclei from still differentiating cells. Eight-day-old rats were injected intracisternally with [14C]thymidine and [3H]tryptophan and the 3H/14C ratio of the chromatin was followed for a period of 33 days. It was found that in the fraction of differentiated cells this ratio remained unchanged, showing the presence of metabolically stable NHCP. At the same time in the fraction containing nondifferentiated cells a substantial part of the NHCP was turned over, which was indicated by a sharp decrease in their 3H/14C ratio with time.
在来自完全分化细胞的大鼠脑细胞核以及仍在分化的细胞的细胞核中研究了非组蛋白染色体蛋白（NHCP）的代谢行为。给8日龄大鼠脑池内注射[14C]胸腺嘧啶核苷和[3H]色氨酸，并跟踪染色质的3H/14C比率33天。结果发现，在分化细胞部分，该比率保持不变，表明存在代谢稳定的NHCP。与此同时，在含有未分化细胞的部分中，相当一部分NHCP发生了周转，这表现为它们的3H/14C比率随时间急剧下降。
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