DiVecchia L, Somerson N L
Appl Microbiol. 1973 Sep;26(3):298-302. doi: 10.1128/am.26.3.298-302.1973.
The reduction of tetrazolium was used to assay the metabolic capability of developing Mycoplasma pneumoniae cultures on glass. Generally, the amount of tetrazolium reduced correlates with the amount of growth as measured by protein. Until a culture enters the late phase of the growth cycle, the drop in pH of culture medium provides similar information. In this last stage of growth, protein appears to be leveling. The pH continues to fall, but tetrazolium reducing activity decreases. Thus, considering the entire M. pneumoniae growth cycle, formazan production is a more reliable measure of metabolic capability of the organisms than either protein or pH. The reduction of tetrazolium provides a quantitative means of assessing enzymatic activities of glass-adherent M. pneumoniae.
采用四氮唑还原法测定在玻璃上培养的肺炎支原体的代谢能力。一般来说，四氮唑的还原量与通过蛋白质测量的生长量相关。在培养物进入生长周期后期之前，培养基pH值的下降提供了类似的信息。在生长的最后阶段，蛋白质含量似乎趋于稳定。pH值继续下降，但四氮唑还原活性降低。因此，考虑到肺炎支原体的整个生长周期，甲臜生成比蛋白质或pH值更能可靠地衡量该生物体的代谢能力。四氮唑的还原提供了一种定量评估玻璃黏附性肺炎支原体酶活性的方法。
