Momparler R L, Veselý J, Momparler L F, Rivard G E
Cancer Res. 1979 Oct;39(10):3822-7.
The biochemical and biological effects of the combination of 5-aza-2'-deoxycytidine (5-aza-dCyd) and 3-deazauridine (3-DU) on L1210 leukemic cells and EMT6 tumor cells were investigated. The cytotoxic action of 5-aza-dCyd and 3-DU on both L1210 and EMT6 cells in vitro was synergistic when these agents were used in combination. The combination of 5-aza-dCyd and 3-DU produced a greater inhibition of in vitro growth of L1210 and EMT6 cells than did either agent alone. The in vivo antineoplastic activity of this combination was synergistic with respect to the increased survival time of BALB/c x DBA/2 F1 mice with L1210 leukemia. 3-DU, an agent that reduces the intracellular pool size of cytosine nucleotides, stimulated the incorporation of [3H]-5-aza-dCyd into DNA of both L1210 and EMT6 cells, suggesting that the synergistic action of this combination is related to the increased incorporation of 5-aza-dCyd in the presence of 3-DU.
研究了5-氮杂-2'-脱氧胞苷（5-aza-dCyd）与3-脱氮尿苷（3-DU）联合使用对L1210白血病细胞和EMT6肿瘤细胞的生化及生物学效应。当联合使用时，5-aza-dCyd和3-DU对L1210和EMT6细胞的体外细胞毒性作用具有协同性。5-aza-dCyd与3-DU联合使用对L1210和EMT6细胞体外生长的抑制作用比单独使用任何一种药物都更强。就延长携带L1210白血病的BALB/c×DBA/2 F1小鼠的存活时间而言，该联合用药的体内抗肿瘤活性具有协同性。3-DU是一种可减少胞嘧啶核苷酸细胞内池大小的药物，它刺激了[3H]-5-aza-dCyd掺入L1210和EMT6细胞的DNA中，这表明该联合用药的协同作用与在3-DU存在的情况下5-aza-dCyd掺入增加有关。
