来自青霉属分生孢子的病毒颗粒。
Virus particles from conidia of Penicillium species.
作者信息
Sansing G A, Detroy R W, Freer S N, Hesseltine C W
出版信息
Appl Microbiol. 1973 Dec;26(6):914-8. doi: 10.1128/am.26.6.914-918.1973.
Virus particles and their component double-stranded ribonucleic acid (dsRNA) have been isolated from conidia and mycelia of certain Penicillium species. The conidia and mycelia of P. stoloniferum NRRL 5267 contained 75 and 85 mug of dsRNA/g (dry weight), respectively. Of the total dsRNA released from NRRL 5267 conidia, 10% was nonencapsulated. Conidia of P. brevi-compactum NRRL 5260 and P. chrysogenum Q-176 contained 2 and 120 mug of dsRNA/g (dry weight), respectively, whereas mycelium from the two species contained 3 and 95 mug of dsRNA/g (dry weight), respectively. No viruses were isolated from conidia or mycelia of P. stoloniferum NRRL 859. A method is described for disruption of both conidia and mycelia. The technique facilitates the isolation and characterization of fungal viruses and their component dsRNA and also potentiates surveying of fungal isolates for the presence of virus.
已从某些青霉属物种的分生孢子和菌丝体中分离出病毒颗粒及其组成成分双链核糖核酸（dsRNA）。匍枝根霉NRRL 5267的分生孢子和菌丝体分别含有75和85微克dsRNA/克（干重）。从NRRL 5267分生孢子中释放的总dsRNA中，10%是非包裹性的。短密青霉NRRL 5260和产黄青霉Q-176的分生孢子分别含有2和120微克dsRNA/克（干重），而这两个物种的菌丝体分别含有3和95微克dsRNA/克（干重）。未从匍枝根霉NRRL 859的分生孢子或菌丝体中分离出病毒。描述了一种破坏分生孢子和菌丝体的方法。该技术有助于真菌病毒及其组成dsRNA的分离和鉴定，也有助于检测真菌分离物中是否存在病毒。
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