Hershey S J, Simon T W, Baste C
J Histochem Cytochem. 1975 Apr;23(4):271-82. doi: 10.1177/23.4.47870.
A cytochemical technique for localizing cytochrome oxidase activity, based upon the oxidative polymerization of 3,3-diaminobenzidine (DAB) to an osmiophilic reaction product, has been employed to test the possibility of extramitochondrial cytochrome oxidase in gastric mucosa. Deposition of reaction product was found to be exclusively localized within mitochondris; in particular, no reaction product was observed at the apical plasma membrane. Measurements of the effect of DAB on acid secretion revealed a biphasic action consisting of an initial stimulation followed by inhibition. The stimulation of secretion of DAB is interpreted to indicate that DAB is oxidized via a pathway which is linked to the secretory process. The combined cytochemical and physiological measurements provide evidence that the metabolic energy supply for acid secreation is derived from mitochondrial reactions. The results are discussed in relation to current models for the coupling between acid secreation and oxidative metabolism.
一种基于3,3 - 二氨基联苯胺（DAB）氧化聚合成嗜锇反应产物来定位细胞色素氧化酶活性的细胞化学技术，已被用于检测胃黏膜中线粒体外细胞色素氧化酶的可能性。发现反应产物的沉积仅局限于线粒体；特别是在顶端质膜未观察到反应产物。对DAB对胃酸分泌影响的测量显示出一种双相作用，包括最初的刺激随后是抑制。DAB对分泌的刺激被解释为表明DAB通过与分泌过程相关的途径被氧化。细胞化学和生理学测量相结合提供了证据，表明胃酸分泌的代谢能量供应来自线粒体反应。结合当前胃酸分泌与氧化代谢偶联模型对结果进行了讨论。
