在限定培养基中生产巴氏芽孢梭菌。
Production of Clostridium pasteurianum in a defined medium.
作者信息
Sargeant K, Ford J W, Longyear V M
出版信息
Appl Microbiol. 1968 Feb;16(2):296-300. doi: 10.1128/am.16.2.296-300.1968.
A method for the growth of Clostridium pasteurianum in a 140-liter (total capacity) stainless-steel vessel is described. By preventing the pH value from falling below 5.6, the growth of cultures was prolonged. Larger amounts of the carbon source (sucrose) and the nitrogen source (ammonium ion) were supplied and consumed, and cell yields of up to 5.56 g (dry weight) per liter were obtained. The highest cell yield previously reported was 1.7 g (dry weight) per liter obtained under nitrogen-fixing conditions in 500-ml cultures. The ferredoxin content of the cells was comparable with that obtained by earlier workers.
描述了一种在140升（总容量）不锈钢容器中培养巴氏梭菌的方法。通过防止pH值降至5.6以下，培养物的生长得以延长。提供并消耗了更多量的碳源（蔗糖）和氮源（铵离子），每升获得了高达5.56克（干重）的细胞产量。先前报道的最高细胞产量是在500毫升培养物的固氮条件下每升获得1.7克（干重）。细胞的铁氧化还原蛋白含量与早期研究者所获得的相当。
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