Sharp K, Green M H
J Bacteriol. 1968 Jul;96(1):111-6. doi: 10.1128/jb.96.1.111-116.1968.
The leucine auxotroph Escherichia coli 2961 exhibited stringent control of net ribonucleic acid (RNA) synthesis during amino acid starvation. After leucine was exhausted from the medium, the rate of uracil incorporation into RNA rapidly decreased to 2 to 4% of the prestarvation value. Infection of the starved cells with T2 phage stimulated uracil incorporation to a level equivalent to that of unstarved, T2-infected cells. The RNA synthesized during leucine starvation of the T2-infected cells consisted of T2 and E. coli messenger RNA, but stable ribosomal RNA (23S and 16S) did not appear to be synthesized. It is concluded that one or more T2-specific proteins are required to shut off host messenger RNA synthesis. Furthermore, transcription of E. coli and T2 deoxyribonucleic acid is not necessarily coupled to the translation of messenger RNA during stringent control of net RNA synthesis.
亮氨酸营养缺陷型大肠杆菌2961在氨基酸饥饿期间对净核糖核酸（RNA）合成表现出严格控制。当培养基中的亮氨酸耗尽后，尿嘧啶掺入RNA的速率迅速降至饥饿前值的2%至4%。用T2噬菌体感染饥饿细胞会刺激尿嘧啶掺入，使其达到与未饥饿的、T2感染细胞相当的水平。在T2感染细胞亮氨酸饥饿期间合成的RNA由T2和大肠杆菌信使RNA组成，但稳定的核糖体RNA（23S和16S）似乎未被合成。得出的结论是，关闭宿主信使RNA合成需要一种或多种T2特异性蛋白质。此外，在净RNA合成的严格控制期间，大肠杆菌和T2脱氧核糖核酸的转录不一定与信使RNA的翻译相偶联。
