转化表型在“上皮样”细胞系中的条件性表达。

Conditional expression of the transformed phenotype in an "epithelial-like" cell line.

作者信息

Naha P M, Sorrentino R

出版信息

Cell Biol Int Rep. 1979 Aug;3(5):421-30. doi: 10.1016/0309-1651(79)90003-1.

Abstract

A subclone of ts 14/MNU/2, derived from a heteroploid (epithelial) monkey kidney cell line of BSC-1 and transformed in vitro by methylnitrosourea, expressed the transformed phenotype only at the elevated temperature of 39.5(0)C. Compared to the growth characteristics at 33(0)C, the transformed property was exhibited in the (1) high efficiency of plating at low cell densities, (2) colony morphology, (3) growth in absence of serum and (4) alterations in the quality and the quantity of chromosomal proteins in presence of low concentrations of serum. Such experimental systems may provide an effective control for studying molecular mechanisms related to transformation and neoplasia.

摘要

ts 14/MNU/2亚克隆源自异倍体（上皮）猴肾细胞系BSC-1，经甲基亚硝基脲体外转化，仅在39.5℃的高温下表现出转化表型。与33℃时的生长特性相比，转化特性体现在：（1）低细胞密度下的高接种效率；（2）集落形态；（3）无血清生长；（4）低浓度血清存在时染色体蛋白质的质量和数量改变。此类实验系统可为研究与转化和肿瘤形成相关的分子机制提供有效对照。