Boyle S M, Cohen P S
J Bacteriol. 1968 Oct;96(4):1266-72. doi: 10.1128/jb.96.4.1266-1272.1968.
Escherichia coli treated with chloramphenicol (CM) accumulated ribonucleic acid (RNA) in the absence of protein synthesis. The accumulated RNA (CM-RNA) was largely ribosomal (23S and 16S) and soluble (4S). The stability of CM-RNA depended upon the incubation conditions following the removal of CM. Thus, conditions which allowed the complete recovery of cultures from CM inhibition resulted in only a 30% loss of CM-RNA. The addition of proflavine to recovering cultures, which prevented further RNA synthesis, also resulted in about 30 to 35% degradation of CM-RNA. However, when RNA synthesis was inhibited by starving the recovering cultures for the required amino acid, histidine, 55% of the CM-RNA was degraded. The decreased stability of CM-RNA in histidine-starved cultures appeared to be due specifically to the intracellular buildup of putrescine. Under the above conditions of incubation, that RNA which was stable sedimented in sucrose gradients as 23S, 16S, and 4S RNA. It is suggested that intracellular putrescine plays a role in the stability of ribosomal RNA accumulated during CM treatment.
用氯霉素（CM）处理的大肠杆菌在缺乏蛋白质合成的情况下积累了核糖核酸（RNA）。积累的RNA（CM-RNA）主要是核糖体RNA（23S和16S）以及可溶性RNA（4S）。CM-RNA的稳定性取决于去除CM后的培养条件。因此，能使培养物从CM抑制中完全恢复的条件下，CM-RNA仅损失30%。向正在恢复的培养物中添加原黄素，阻止了进一步的RNA合成，也导致CM-RNA降解约30%至35%。然而，当通过使正在恢复的培养物缺乏所需氨基酸组氨酸来抑制RNA合成时，55%的CM-RNA被降解。在组氨酸饥饿的培养物中CM-RNA稳定性降低似乎特别归因于腐胺在细胞内的积累。在上述培养条件下，稳定的RNA在蔗糖梯度中沉降为23S、16S和4S RNA。有人提出细胞内腐胺在CM处理期间积累的核糖体RNA的稳定性中起作用。
