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围绕生枝动胶菌的菌胶团基质的精细结构和组成

Fine structure and composition of the zoogloeal matrix surrounding Zoogloea ramigera.

作者信息

Friedman B A, Dugan P R, Pfister R M, Remsen C C

出版信息

J Bacteriol. 1968 Dec;96(6):2144-53. doi: 10.1128/jb.96.6.2144-2153.1968.

DOI:10.1128/jb.96.6.2144-2153.1968
PMID:4881706
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC252569/
Abstract

The fingerlike projections, which have been considered to be characteristic of the genus Zoogloea, appear to consist of generally globular packets of cells, each surrounded by capsular matrix. The individual packets which surround Z. ramigera 115 cells appear to adhere one to another by intermeshed fibrils that measure 2 to 5 nm in diameter. The fibril polymer appears to be polyglucose that is susceptible to cellulase. The polymer resembles cellulose in several respects, with the exception that it is soluble in 1 n NaOH. Although Z. ramigera I-16-M does not possess an observable zoogloeal matrix when viewed under a light microscope, chemical data indicate that it does produce cellulase-susceptible polymer. Fibrils can be observed under the electron microscope, which are resistant to 1 n NaOH and may be cellulose. Less polymer fibrils are observed around isolate I-16-M cells than around isolate 115 cells; the inability to observe the I-16-M material as a zoogloeal matrix under light microscopy may be due to lack of sufficient amount of polymer.

摘要

这些指状突起被认为是动胶菌属的特征,似乎由通常呈球形的细胞包组成,每个细胞包都被荚膜基质包围。围绕枝状动胶菌115细胞的单个细胞包似乎通过直径为2至5纳米的相互交织的纤维彼此粘附。纤维聚合物似乎是易受纤维素酶作用的聚葡萄糖。该聚合物在几个方面类似于纤维素,不同之处在于它可溶于1N氢氧化钠。尽管在光学显微镜下观察时,枝状动胶菌I-16-M没有可观察到的动胶菌基质,但化学数据表明它确实产生了易受纤维素酶作用的聚合物。在电子显微镜下可以观察到对1N氢氧化钠有抗性且可能是纤维素的纤维。在分离株I-16-M细胞周围观察到的聚合物纤维比在分离株115细胞周围少;在光学显微镜下无法将I-16-M物质观察为动胶菌基质可能是由于聚合物数量不足。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08d4/252569/aae1913b5393/jbacter00398-0295-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08d4/252569/e7452dabe25c/jbacter00398-0290-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08d4/252569/6829602439e3/jbacter00398-0291-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08d4/252569/3659e83a33e0/jbacter00398-0292-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08d4/252569/bf1e9f9d13ab/jbacter00398-0293-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08d4/252569/aae1913b5393/jbacter00398-0295-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08d4/252569/e7452dabe25c/jbacter00398-0290-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08d4/252569/6829602439e3/jbacter00398-0291-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08d4/252569/3659e83a33e0/jbacter00398-0292-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08d4/252569/bf1e9f9d13ab/jbacter00398-0293-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/08d4/252569/aae1913b5393/jbacter00398-0295-a.jpg

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