Ho P P, Frank B H, Burck P J
Science. 1969 Aug 1;165(3892):510-2. doi: 10.1126/science.165.3892.510.
L-Asparaginase has been crystallized from a partially purified extract of Escherichia coli B. The crystalline enzyme is homogeneous, as judged by analytical polyacrylamidegel electrophoresis and sedimentation behavior. This enzyme preparation is active in preventing lymphoma in mice and also has low glutaminase activity.
L-天冬酰胺酶已从大肠杆菌B的部分纯化提取物中结晶出来。通过分析聚丙烯酰胺凝胶电泳和沉降行为判断,结晶酶是纯一的。这种酶制剂在预防小鼠淋巴瘤方面具有活性,并且谷氨酰胺酶活性较低。