暗场电子显微镜下的大分子精细结构

Biophys J. 1969 Sep;9(9):1144-9. doi: 10.1016/S0006-3495(69)86441-6.

The potential of dark field electron microscopy for examining details of unstained unshadowed macromolecules has been studied. Images produced by scattered electrons show sufficient contrast and resolution to reveal the helical nature of short sections of DNA or the cleft containing the active site in ribonuclease. Spreads of transfer RNA were observed to contain many trifoliate structures.

已经研究了暗场电子显微镜在检查未染色、未投影大分子细节方面的潜力。由散射电子产生的图像显示出足够的对比度和分辨率，以揭示DNA短片段的螺旋性质或核糖核酸酶中包含活性位点的裂隙。观察到转移RNA的铺展含有许多三叶形结构。