Farnsworth W E, Wilks J W
Prostaglandins. 1975 Jan;9(1):67-74. doi: 10.1016/s0090-6980(75)80118-3.
Earlier work had shown that the lactogen, LTH and HPL, foster testosterone binding by the prostate. This study was undertaken to see if prostaglandin F2alpha would oppose the effect of the lactogen on the prostate as it does the luteotrophic action of the hormone on the corpus luteum. When it was found instead that the PGF increases steroid binding and that its interaction with lactogen was neither antagonistic nor additive, attention was directed to further characterization of prostaglandin's effect. A dosage/response study of F2alpha alone showed that concentrations of 4 ng/ml and 40 ng/ml increased binding but that 400 ng/ml did not. Glands with stromal hyperplasia and/or inflammation were not responsive than those with epithelial hyperplasia. Assays of water extracts of the tissue revealed concentrations of about 340 ng of F2alpha per gram fresh weight and that the concentration varied inversely as the beta-glucuronidase activity. If the enzyme level is considered an index of the epithelial cell density within the specimen, the inverse relationship suggest a non-epithelial (stromal) site of prostaglandin concentration.
早期的研究表明，催乳素、促乳素和人胎盘催乳素可促进前列腺对睾酮的结合。本研究旨在探究前列腺素F2α是否会像它对激素对黄体的促黄体作用那样，拮抗催乳素对前列腺的作用。然而，研究发现前列腺素F2α会增加类固醇结合，并且它与催乳素的相互作用既不是拮抗作用也不是相加作用，于是研究重点转向对前列腺素作用的进一步表征。单独使用F2α的剂量/反应研究表明，4纳克/毫升和40纳克/毫升的浓度会增加结合，但400纳克/毫升则不会。与上皮增生的腺体相比，有基质增生和/或炎症的腺体反应不明显。对组织水提取物的检测显示，每克鲜重中约含有340纳克F2α，且该浓度与β-葡萄糖醛酸酶活性呈反比。如果将酶水平视为标本中上皮细胞密度的指标，这种反比关系表明前列腺素的浓度位于非上皮（基质）部位。
