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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Krishna R V, Krishnaswamy P R, Rao D R

Biochem J. 1971 Oct;124(5):905-13. doi: 10.1042/bj1240905.

Cell-free extracts of Escherichia coli K12 catalyse the synthesis of N-acetyl-l-phenylalanine from acetyl-CoA and l-phenylalanine. 2. The acetyl-CoA-l-phenylalanine alpha-N-acetyltransferase was purified 160-fold from cell-free extracts. 3. The enzyme has a pH optimum of 8 and catalyses the acetylation of l-phenylalanine. Other l-amino acids such as histidine and alanine are acetylated at slower rates. 4. A transacylase was also purified from E. coli extracts and its substrate specificity studied. 5. The properties of both these enzymes were compared with those of other known amino acid acetyltransferases and transacylases.

大肠杆菌K12的无细胞提取物可催化由乙酰辅酶A和L-苯丙氨酸合成N-乙酰-L-苯丙氨酸。2. 从无细胞提取物中纯化出乙酰辅酶A-L-苯丙氨酸α-N-乙酰转移酶，纯化倍数为160倍。3. 该酶的最适pH为8，催化L-苯丙氨酸的乙酰化反应。其他L-氨基酸如组氨酸和丙氨酸的乙酰化速率较慢。4. 还从大肠杆菌提取物中纯化出一种转酰基酶，并研究了其底物特异性。5. 将这两种酶的特性与其他已知的氨基酸乙酰转移酶和转酰基酶的特性进行了比较。

Krishna R V, Krishnaswamy P R, Rao D R

Biochem J. 1971 Oct;124(5):905-13. doi: 10.1042/bj1240905.

Cell-free extracts of Escherichia coli K12 catalyse the synthesis of N-acetyl-l-phenylalanine from acetyl-CoA and l-phenylalanine. 2. The acetyl-CoA-l-phenylalanine alpha-N-acetyltransferase was purified 160-fold from cell-free extracts. 3. The enzyme has a pH optimum of 8 and catalyses the acetylation of l-phenylalanine. Other l-amino acids such as histidine and alanine are acetylated at slower rates. 4. A transacylase was also purified from E. coli extracts and its substrate specificity studied. 5. The properties of both these enzymes were compared with those of other known amino acid acetyltransferases and transacylases.

大肠杆菌K12的无细胞提取物可催化由乙酰辅酶A和L-苯丙氨酸合成N-乙酰-L-苯丙氨酸。2. 从无细胞提取物中纯化出乙酰辅酶A-L-苯丙氨酸α-N-乙酰转移酶，纯化倍数为160倍。3. 该酶的最适pH为8，催化L-苯丙氨酸的乙酰化反应。其他L-氨基酸如组氨酸和丙氨酸的乙酰化速率较慢。4. 还从大肠杆菌提取物中纯化出一种转酰基酶，并研究了其底物特异性。5. 将这两种酶的特性与其他已知的氨基酸乙酰转移酶和转酰基酶的特性进行了比较。