Rowley D B, Levinson H S
J Bacteriol. 1967 Mar;93(3):1017-22. doi: 10.1128/jb.93.3.1017-1022.1967.
Spores of Bacillus megaterium QM B1551 treated with thioglycolate (0.4 m, pH 2.6) at 50 C for 30 min remained refractile, but they became stainable, lysozymesensitive, and nonviable, and they lost dipicolinic acid (DPA). The loss of DPA and of viability were functions of the time and temperature of exposure to thioglycolate. Spores treated with thioglycolate at a lower temperature and for a shorter time (30 C, 5 min) retained DPA, viability, and nonstainability. Although these spores also retained their resistance to gamma radiation and to lysozyme, they lost thermo-resistance. Their percentage of germination over a 2-hr period in glucose was markedly reduced. Germinability and heat resistance were restored by exogenous cations, suggesting that the thioglycolate treatment (30 C, 5 min) resulted in the loss of spore ions essential for normal germination in glucose and for heat resistance.
用巯基乙酸盐（0.4 m，pH 2.6）在50℃处理巨大芽孢杆菌QM B1551的孢子30分钟后，它们仍具折光性，但变得可染色、对溶菌酶敏感且无活力，并且失去了吡啶二羧酸（DPA）。DPA的丧失和活力的丧失是暴露于巯基乙酸盐的时间和温度的函数。在较低温度和较短时间（30℃，5分钟）下用巯基乙酸盐处理的孢子保留了DPA、活力和不可染色性。尽管这些孢子也保留了对γ辐射和溶菌酶的抗性，但它们失去了热抗性。它们在葡萄糖中2小时内的萌发率显著降低。外源阳离子可恢复其萌发能力和耐热性，这表明巯基乙酸盐处理（30℃，5分钟）导致了孢子中对葡萄糖正常萌发和耐热性至关重要的离子丧失。
