Suppr超能文献

谷胱甘肽对核糖核酸酶的作用。

Effect of glutathione on ribonuclease.

作者信息

Kim S, Paik W K

出版信息

Biochem J. 1968 Feb;106(3):707-10. doi: 10.1042/bj1060707.

DOI:10.1042/bj1060707
PMID:4966474
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1198562/
Abstract

GSH, but not GSSG, inhibits the reactivation by phosphate ion of ribonuclease activity inactivated by urea or guanidine. The effects of GSH are rather slow and pretreatment of ribonuclease with urea is a requisite for the inhibitory action of GSH on enzyme reactivation. GSH is more effective in urea than in guanidine and its action is greatly enhanced by EDTA. An optimum pH of about 9.0 was found for the inhibitory effect of GSH. Titration of the thiol groups formed after inactivation of ribonuclease by GSH strongly suggests that the reduction of only one disulphide linkage is involved. The reduction of this bond is sufficient to completely abolish the enzymic activity.

摘要

谷胱甘肽（GSH）而非氧化型谷胱甘肽（GSSG）可抑制被尿素或胍灭活的核糖核酸酶活性被磷酸根离子重新激活。谷胱甘肽的作用相当缓慢，并且用尿素对核糖核酸酶进行预处理是谷胱甘肽对酶再激活产生抑制作用的必要条件。谷胱甘肽在尿素中比在胍中更有效，并且其作用会因乙二胺四乙酸（EDTA）而大大增强。发现谷胱甘肽的抑制作用的最佳pH约为9.0。对谷胱甘肽使核糖核酸酶失活后形成的巯基进行滴定，有力地表明仅涉及一个二硫键的还原。该键的还原足以完全消除酶活性。

相似文献

1
Effect of glutathione on ribonuclease.谷胱甘肽对核糖核酸酶的作用。
Biochem J. 1968 Feb;106(3):707-10. doi: 10.1042/bj1060707.
2
The reaction of 4-(p-trimethylammoniumphenylazo)-2-hydroxymercuriphenol with the thiol groups of proteins.4-（对-三甲基铵苯基偶氮）-2-羟基汞苯酚与蛋白质硫醇基团的反应
Biochim Biophys Acta. 1971 May 25;236(2):487-96. doi: 10.1016/0005-2795(71)90229-7.
3
Influence of glutathione on the reactivation of enzymes containing cysteine or cystine.谷胱甘肽对含半胱氨酸或胱氨酸的酶再活化的影响。
Hoppe Seylers Z Physiol Chem. 1983 Jul;364(7):813-20. doi: 10.1515/bchm2.1983.364.2.813.
4
ALTERATIONS IN THE PHYSICAL AND ENZYMIC PROPERTIES OF ALPHA-CHYMOTRYPSIN IN UREA AND GUANIDINIUM CHLORIDE.α-胰凝乳蛋白酶在尿素和氯化胍中物理及酶学性质的改变
Biochemistry. 1964 Nov;3:1635-43. doi: 10.1021/bi00899a006.
5
The reactivity of the disulphide bonds of bovine pancreatic ribonuclease with glutathione.牛胰核糖核酸酶二硫键与谷胱甘肽的反应活性
Biochem J. 1965 Sep;96(3):890-4. doi: 10.1042/bj0960890.
6
THE EFFECT OF PYRIDINE DERIVATIVES ON THE ACTIVITY OF CRYSTALLINE RIBONUCLEASE.
Biochim Biophys Acta. 1964 Jun 22;87:181-7.
7
Effects of the solvent medium on polyvalylribonuclease aggregation.溶剂介质对聚缬氨酰核糖核酸酶聚集的影响。
Biochemistry. 1968 Apr;7(4):1506-13. doi: 10.1021/bi00844a037.
8
Proteins as random coils. II. Hydrogen ion titration curve of ribonuclease in 6 M guanidine hydrochloride.
J Am Chem Soc. 1967 Feb 15;89(4):742-9. doi: 10.1021/ja00980a003.
9
Phosphorylation coupled to oxidation of thiol groups (GSH) by cytochrome c with disulfide (GSSG) as an essential catalyst. I. Demonstration of ADP formation from AMP and HPO4 2-.
Biochem Biophys Res Commun. 1970 Jul 27;40(2):360-8. doi: 10.1016/0006-291x(70)91018-1.
10
PURIFICATION OF TWO ENDONUCLEASES FROM POTATO TUBERS.从马铃薯块茎中纯化两种核酸内切酶。
Biochim Biophys Acta. 1965 Apr 19;95:652-62.

本文引用的文献

1
The relation of structure to enzymatic activity in ribonuclease.核糖核酸酶中结构与酶活性的关系。
Ann N Y Acad Sci. 1959 Sep 4;81:542-69. doi: 10.1111/j.1749-6632.1959.tb49336.x.
2
PURIFICATION AND PROPERTIES OF A MICROSOMAL ENZYME SYSTEM CATALYZING THE REACTIVATION OF REDUCED RIBONUCLEASE AND LYSOZYME.催化还原型核糖核酸酶和溶菌酶再活化的微粒体酶系统的纯化及性质
J Biol Chem. 1964 May;239:1406-10.
3
Regeneration of enzyme activity by air oxidation of reduced subtilisin-modified ribonuclease.通过对还原的枯草杆菌蛋白酶修饰的核糖核酸酶进行空气氧化来恢复酶活性。
J Biol Chem. 1961 Feb;236:422-4.
4
Studies on the reduction and re-formation of protein disulfide bonds.蛋白质二硫键的还原与重新形成研究。
J Biol Chem. 1961 May;236:1361-3.
5
The relationship of disulfide bonds and activity in ribonuclease.核糖核酸酶中二硫键与活性的关系。
J Biol Chem. 1959 Jul;234(7):1711-3.
6
The reductive cleavage of disulfide bonds and its application to problems of protein structure.
Biochim Biophys Acta. 1959 Feb;31(2):417-26. doi: 10.1016/0006-3002(59)90016-2.
7
The correlation of ribonuclease activity with specific aspects of tertiary structure.核糖核酸酶活性与三级结构特定方面的相关性。
Biochim Biophys Acta. 1957 Dec;26(3):502-12. doi: 10.1016/0006-3002(57)90096-3.
8
Reductive cleavage of disulfide bridges in ribonuclease.核糖核酸酶中二硫键的还原裂解
Science. 1957 Apr 12;125(3250):691-2. doi: 10.1126/science.125.3250.691.
9
Studies on the specificity and mechanism of action of hepatic glutathione-insulin transhydrogenase.
J Biol Chem. 1966 Aug 10;241(15):3561-70.

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验