在恒定pH值下通过滴定法测定碳酸酐酶
Assay of carbonic anhydrase by titration at constant pH.
作者信息
McIntosh J E
出版信息
Biochem J. 1968 Sep;109(2):203-7. doi: 10.1042/bj1090203.
Abstract
- A method is described for measuring accurately the initial velocity of the hydration reaction catalysed by the enzyme carbonic anhydrase (EC 4.2.1.1); the method depends on the titration of H(+) ions at constant pH. 2. Human erythrocyte carbonic anhydrase, isoenzyme C, was used to illustrate the method. Under the experimental conditions employed (0 degrees , pH7.0, in the presence of 45mm-sodium chloride and 5mm-sodium phosphate) isoenzyme C obeyed the Michaelis equation over the range of substrate concentration 1-16mm-carbon dioxide. The kinetic constants found were: K(m)=8.2mm; V/[E(0)]=5.0x10(4) sec.(-1).
摘要
- 本文描述了一种精确测量由碳酸酐酶（EC 4.2.1.1）催化的水合反应初始速度的方法；该方法基于在恒定pH下对H(+)离子的滴定。2. 使用人红细胞碳酸酐酶同工酶C来说明该方法。在所采用的实验条件下（0摄氏度，pH7.0，存在45mM氯化钠和5mM磷酸钠），同工酶C在底物浓度1-16mM二氧化碳范围内符合米氏方程。得到的动力学常数为：K(m)=8.2mM；V/[E(0)]=5.0x10(4) 秒(-1)。
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本文引用的文献
1
The manometric determination of the activity of carbonic anhydrase under varied conditions.
Biochem J. 1946;40(2):309-19. doi: 10.1042/bj0400309.
2
The catalytic effect of buffers on the reaction CO(2)+H(2)Oright harpoon over left harpoonH(2)CO(3).
Biochem J. 1938 Nov;32(11):2049-69. doi: 10.1042/bj0322049.
3
A re-investigation of the question of activators of carbonic anhydrase.
Biochem J. 1951 Apr;48(4):495-503. doi: 10.1042/bj0480495.
4
KINETIC STUDIES OF HUMAN CARBONIC ANHYDRASES B AND C.
J Biol Chem. 1964 Aug;239:2539-44.
5
CARBONIC ANHYDRASES FROM HUMAN ERYTHROCYTES. PREPARATION AND PROPERTIES OF TWO ENZYMES.
J Biol Chem. 1964 Apr;239:1065-78.
6
RATE OF HYDRATION OF CARBON DIOXIDE AND DEHYDRATION OF CARBONIC ACID AT 25 DEGREES.
J Biol Chem. 1963 Oct;238:3502-7.
7
THE KINETICS OF THE HYDRATION OF CARBON DIOXIDE AT 25 DEGREES.
J Biol Chem. 1963 Oct;238:3499-501.
8
The activity of concentrated solutions of carbonic anhydrase.
Biochim Biophys Acta. 1963 Jan 8;67:31-41. doi: 10.1016/0006-3002(63)91794-3.
9
On the determination of carbonic anhydrase in biological material.
Scand J Clin Lab Invest. 1961;13:416-7.
10
Nature of the inhibition of carbonic anhydrase by acetazolamide and benzthiazide.
J Pharmacol Exp Ther. 1961 Mar;131:271-4.
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