Bunn H F, Meriwether W D, Balcerzak S P, Rucknagel D L
J Clin Invest. 1972 Nov;51(11):2984-7. doi: 10.1172/JCI107125.
Oxygen equilibrium was determined on hemoglobin of individuals both heterozygous and homozygous for hemoglobin E. The whole blood oxyhemoglobin dissociation curve of AE blood was identical to that of normal AA blood. E hemoglobin, isolated by diethylaminoethyl Sephadex and carboxymethyl cellulose column chromatography, had oxygen affinity, heme-heme interaction, and Bohr effect identical to those of hemoglobin A prepared from the same column. Furthermore, the two hemoglobins had equal reactivity with 2,3-diphosphoglycerate. Phosphate-free hemolysates of blood from E and A homozygotes also had identical oxygen saturation curves. These results do not confirm earlier reports that hemoglobin E has an abnormally low oxygen affinity.
对血红蛋白E杂合子和纯合子个体的血红蛋白进行了氧平衡测定。AE血型全血的氧合血红蛋白解离曲线与正常AA血型的相同。通过二乙氨基乙基葡聚糖和羧甲基纤维素柱色谱法分离得到的E型血红蛋白,其氧亲和力、血红素-血红素相互作用以及玻尔效应与从同一柱制备的血红蛋白A相同。此外,这两种血红蛋白与2,3-二磷酸甘油酸的反应性相等。E型和A型纯合子血液的无磷酸盐溶血产物也具有相同的氧饱和度曲线。这些结果并不证实早期关于血红蛋白E具有异常低氧亲和力的报道。