Bowe W J, Mandelkern L
Science. 1971 Jul 16;173(3993):239-40. doi: 10.1126/science.173.3993.239.
The isometric tension of glycerinated muscle fibers and the adenosine triphosphatase activity of homogenates were determined as a function of the concentration of adenosine triphosphate without the addition of divalent cations. These two phenomena are not parallel; large tensions can be developed with negligible hydrolysis of adenosine triphosphate. It is concluded that the large negative free energy change of the hydrolysis is not required for shortening or development of tension.
在不添加二价阳离子的情况下,测定了甘油处理的肌肉纤维的等长张力以及匀浆的三磷酸腺苷酶活性,二者均作为三磷酸腺苷浓度的函数。这两种现象并不平行;在三磷酸腺苷水解可忽略不计的情况下,仍可产生较大张力。由此得出结论,缩短或产生张力并不需要水解反应的大量负自由能变化。