来自对白纹伊蚊α-鹅膏蕈碱有抗性的细胞突变体的α-鹅膏蕈碱抗性RNA聚合酶II

alpha-Amanitin resistant RNA polymerase II from Aedes albopictus cell mutants resistant to alpha-amanitin.

作者信息

Mento S J, Stollar V

出版信息

Cell Biol Int Rep. 1979 Dec;3(9):725-32. doi: 10.1016/0309-1651(79)90077-8.

Abstract

Spontaneous and EMS-induced alpha-amanitin-resistant Aedes albopictus cells have been isolated and characterized. Two mutant sublines, one of intermediate resistance (alpha A2) and the other highly resistant (Ama18) contained RNA polymerase II activity, the resistance of which in vitro to alpha-amanitin correlated well with the resistance of these cells in vivo. The resistance of these cells to alpha-amanitin can likely be attributed to the presence of an altered RNA polymerase II.

摘要

已分离并鉴定了自发产生的和经紧急医疗服务诱导产生的对α-鹅膏蕈碱具有抗性的白纹伊蚊细胞。两个突变亚系，一个具有中等抗性（αA2），另一个具有高度抗性（Ama18），它们含有RNA聚合酶II活性，其体外对α-鹅膏蕈碱的抗性与这些细胞在体内的抗性密切相关。这些细胞对α-鹅膏蕈碱的抗性可能归因于存在一种改变的RNA聚合酶II。