睾酮 - 雌二醇结合球蛋白在17 - 氧化C19类固醇酶促氧化还原反应中的作用。
Effect of testosterone-estradiol-binding globulin in the enzymic oxidoreduction of 17-oxygenated c19 steroids.
作者信息
Hampl R, Stárka L
出版信息
Horm Res. 1975;6(1):57-64. doi: 10.1159/000178662.
The effect of both testosterone-estradiol-binding globulin (TeBG) and albumin on enzymic oxidoreduction of four 17-oxygenated C19 steroids by bacterial 17beta-hydroxysteroid:NAD oxidoreductase from Pseudomonas testosteroni was investigated. The decreased yields of products under presence of TeBG were found in both directions of reversible enzymic reaction. This finding was unexpected in the case of enzymic reduction in which the opposite effect could be assumed with respect of high affinity of the product but not the substrate to TeBG. Kinetically, the competition between enzyme and binding protein for the substrate occurs at enzymic oxidation, whereas the mechanism resembling non-competitive inhibition operates in the enzymic reduction.
研究了睾酮 - 雌二醇结合球蛋白（TeBG）和白蛋白对睾丸酮假单胞菌的细菌17β - 羟基类固醇：NAD氧化还原酶对四种17 - 氧化C19类固醇进行酶促氧化还原反应的影响。在可逆酶促反应的两个方向上，均发现TeBG存在时产物产率降低。在酶促还原的情况下，这一发现出乎意料，因为就产物而非底物对TeBG的高亲和力而言，可以假定会有相反的效果。从动力学角度来看，酶与结合蛋白对底物的竞争发生在酶促氧化过程中，而在酶促还原过程中，类似非竞争性抑制的机制起作用。
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