Ceska M, Sjödin A V, Grossmüller F
Biochem J. 1971 Jan;121(1):139-43. doi: 10.1042/bj1210139.
The labelling of proteins by the iodine monochloride method was studied by using a mathematical model. The equations used were primarily derived from the mass law equation of the isotopic exchange reaction between [(125)I]iodide and iodine monochloride. For convenient application, all equations were programmed into a computing desk-top calculator. To support the validity of the theoretical model, a series of iodinations of insulin were performed under various labelling conditions. The results of these experiments compare well with the theoretically derived values. Deviations from the theoretical values occurring at molar ratios of [(125)I]iodide to iodine monochloride < 0.1 and > 4.0 are explained and suggestions made about how to prevent them. The mathematical model was used to simulate the isotopic exchange, and the iodination reaction under various conditions, to study (a) the influence of the amount of [(125)I]iodide on the amount of [(125)I]iodine monochloride formed, (b) the influence of the specific radioactivity of [(125)I]iodide on the amount of [(125)I]iodine monochloride formed, and (c) the influence of the specific radioactivity of [(125)I]iodide on the number of millicuries needed for labelling to a desired extent.
采用数学模型研究了用一氯化碘法标记蛋白质的过程。所使用的方程主要源自[(125)I]碘化物与一氯化碘之间同位素交换反应的质量定律方程。为方便应用，将所有方程编入台式计算机进行计算。为验证该理论模型的有效性，在不同标记条件下对胰岛素进行了一系列碘化反应。这些实验结果与理论推导值吻合良好。解释了在[(125)I]碘化物与一氯化碘的摩尔比<0.1和>4.0时出现的与理论值的偏差，并就如何防止这些偏差提出了建议。利用该数学模型模拟了同位素交换以及不同条件下的碘化反应，以研究(a)[(125)I]碘化物的量对形成的[(125)I]一氯化碘量的影响，(b)[(125)I]碘化物的比放射性对形成的[(125)I]一氯化碘量的影响，以及(c)[(125)I]碘化物的比放射性对标记到所需程度所需毫居里数的影响。
