Maickel R P, McFadden D P
Res Commun Chem Pathol Pharmacol. 1979 Oct;26(1):75-83.
Commercially available isobutyl nitrite (iBN), n-butyl nitrite (nBN), sec-butyl nitrite (sBN) and tert-butyl nitrite (tBN) were found to be 63%, 79%, 44% and 96% pure, respectively, by GLC analysis. Pure samples of each compound were synthesized by the action of nitrous acid on each of the corresponding butyl alcohols; GLC analysis indicated greater than 99% purity for each nitrite. In determining LD50 values (mice, i.p.) for each compound, no deaths were observed after 30 min post-administration of sBN or tBN. Gross post-mortem examination suggests the delayed lethality may be due to liver damage. Administration of isobutyl, n-butyl, sec-butyl or tert-butyl alcohols to mice produced similar hepatotoxicity, suggesting that butyl alcohols may play a role in the hepatotoxicity observed after sBN or tBN administration.
通过气相色谱（GLC）分析发现，市售的亚硝酸异丁酯（iBN）、亚硝酸正丁酯（nBN）、亚硝酸仲丁酯（sBN）和亚硝酸叔丁酯（tBN）的纯度分别为63%、79%、44%和96%。通过亚硝酸对相应的丁醇作用合成了每种化合物的纯样品；气相色谱分析表明每种亚硝酸盐的纯度均高于99%。在测定每种化合物的半数致死量（LD50）值（小鼠，腹腔注射）时，注射sBN或tBN后30分钟内未观察到死亡。大体尸检表明，延迟致死可能是由于肝损伤。给小鼠注射异丁醇、正丁醇、仲丁醇或叔丁醇会产生类似的肝毒性，这表明丁醇可能在注射sBN或tBN后观察到的肝毒性中起作用。
