豚鼠细胞的鼠肉瘤病毒转化特性及非生产性豚鼠细胞中一种RNA肿瘤样病毒的激活

Characterization of murine sarcoma virus transformation of guinea pig cells and activation of an RNA tumor-like virus from nonproducer guinea pig cells.

作者信息

Rhim J S, Cho H Y, Duh F G, Vernon M L

出版信息

Bibl Haematol. 1975(40):153-64. doi: 10.1159/000397529.

Abstract

Guinea pig embryo (GEP) cells were transformed in vitro by the Kirsten strain of mouse sarcoma virus (Ki-MSV). The transformed cells were found to release infectious virus continuously and produced high titers of group-specific (gs) complement-fixing (CF) antigen characteristics of the murine sarcoma-leukemia virus complex. Foci of transformed cells were similar in appearance to those obtained with Ki-MSV in mouse and rat cells. The transformed cells produced RNA dependent DNA polymerase and type C virus particles with a density of approximately 1.15 g/ml in sucrose gradients by 3H-uridine labeling. The transformed cells produced tumors when transplanted into newborn guinea pigs. A number of focus-derived clonal lines from Ki-MSV transformed cells were isolated and characterized. All the focus-derived lines were found to be either producers or nonproducers (NP). The NP guinea pig cells produced neither infectious virus nor viral antigens of the murine sarcoma-leukemia virus complex although they were morphologically indistinguishable from virus-releasing MSV transformed GPE lines and produced tumors when transplanted into newborn guinea pigs. However, the sarcoma virus genome could be rescued in these NP cells by cocultivation with "helper" murine leukemia virus (MuLV) releasing GPE cells. Particles resembling guinea pig leukemia virus were activated from guinea pig NP cells or cultured normal guinea pig cells following chemical treatment. These particles were approximately 100 nm in the mature form and had a density of 1.16-1.17 g/ml. They contained RNA dependent DNA polymerase activity.

摘要

豚鼠胚胎（GEP）细胞在体外被小鼠肉瘤病毒（Ki-MSV）的 Kirsten 株转化。发现转化细胞持续释放传染性病毒，并产生高滴度的、具有鼠肉瘤 - 白血病病毒复合物群特异性（gs）补体结合（CF）抗原特征的物质。转化细胞灶在外观上与用 Ki-MSV 在小鼠和大鼠细胞中获得的相似。通过 3H - 尿苷标记，转化细胞在蔗糖梯度中产生密度约为 1.15 g/ml 的 RNA 依赖性 DNA 聚合酶和 C 型病毒颗粒。将转化细胞移植到新生豚鼠体内时会产生肿瘤。从 Ki-MSV 转化细胞中分离并鉴定了许多源自病灶的克隆系。发现所有源自病灶的系要么是病毒产生者，要么是非产生者（NP）。NP 豚鼠细胞既不产生传染性病毒，也不产生鼠肉瘤 - 白血病病毒复合物的病毒抗原，尽管它们在形态上与释放病毒的 MSV 转化 GPE 系无法区分，并且移植到新生豚鼠体内时会产生肿瘤。然而，通过与释放“辅助”鼠白血病病毒（MuLV）的 GPE 细胞共培养，肉瘤病毒基因组可以在这些 NP 细胞中拯救出来。化学处理后，类似豚鼠白血病病毒的颗粒从豚鼠 NP 细胞或培养的正常豚鼠细胞中被激活。这些颗粒成熟形式的大小约为 100 nm，密度为 1.16 - 1.17 g/ml。它们含有 RNA 依赖性 DNA 聚合酶活性。