电鳗（Electrophorus electricus (L.)）电细胞分离膜的细胞化学与冷冻断裂研究

Cytochemistry and freeze-fracture of membranes isolated from the electrocyte of Electrophorus electricus (L.).

作者信息

de Souza W, Benchimol M, Somlo C, Machado R D, Hasson-Voloch A

出版信息

Cell Tissue Res. 1979 Nov;202(2):275-81. doi: 10.1007/BF00232241.

PMID:519706

Abstract

Membranes were isolated from the main electric organ of Electrophorus electricus and studied by means of cytochemistry and freeze-fracture. The membrane fractions consisted of vesicles inside-in as determined by localization of anionic sites using colloidal iron and cationized ferritin particles. The anionic sites were not homogeneously distributed on the surface of the vesicle. Freeze-fracture showed the presence of intramembranous particles associated with either protoplasmic (P) or extracellular (E) faces of the membrane. Regions of the membrane without particles were observed. The results are discussed in relation to the existence of association between intramembranous particles and membrane receptors.

摘要

从电鳗的主要发电器官中分离出膜，并通过细胞化学和冷冻断裂技术进行研究。通过使用胶体铁和阳离子化铁蛋白颗粒定位阴离子位点确定，膜组分由内翻的囊泡组成。阴离子位点在囊泡表面分布不均匀。冷冻断裂显示膜内颗粒与膜的原生质（P）面或细胞外（E）面相关。观察到膜上没有颗粒的区域。结合膜内颗粒与膜受体之间存在关联对结果进行了讨论。