Effects of cyclophosphamide on the prenatal development of the Swiss strain mice.

Groups of pregnant mice were given cyclophosphamide at daily doses from 6.4 to 40.8 mg/kg from the 11th to the 15th day of gravidity. Significantly lower weight and length of fetuses were noted after each of the above dosis as against the controls, while the volume of the amniotic fluid was significantly increased. The number of resorptions rose significantly with the growing dose, the embryotoxicity proving 100-percent effective in both the highest doses. Malformations were followed in live fetuses. Evident malformations resulting from cyclophosphamide application were those of the head (hydrocephalus, exencephalus, exophthalmus, median cleft palate, micrognathia), of the extremities (syndactylia, polydactylia), caudal malformation (stunted and crooked tail) and an overall developmental retardation of the fetuses. Skeletal anomalies comprised synostosis, retarded ossification, deficiency or redundance of some bones of the head, trunk and limbs.