Heatfield B M, Sanefuji H, Trump B F
Scan Electron Microsc. 1979(3):645-56.
Morphologic responses of neoplastic human prostate to long-term explant culture were monitored at serial intervals by LM, TEM and SEM, and compared to normal prostate. Explants were cultured at 37 degrees C in CMRL-1066 supplemented with fetal calf serum and antibiotics. At 0-time culture, normal prostate of young adult males obtained at immediate autopsy, consisted of glandular spaces and ducts lined by columnar to cuboidal secretory epithelial cells and basal cells embedded in fibromuscular stroma. Neoplastic tissue was obtained surgically by transurethral resection (TUR), and consisted of stroma widely infiltrated by well-to moderately-differentiated tumor cells arranged in variable sized, gland-like structures. Secretory activity was evident; basal cells were absent in these glands. During early periods of culture up to several weeks, secretory cells of normal prostate became necrotic. Basal cells remained viable, repopulated acinar structures and epithelialized explant surfaces. At these sites, basal cells, or their derivatives, formed a multicellular epithelium. Exaggerated intercellular spaces separated cells, and synthesis of mucus-like material was seen. Epithelial characteristics included microvilli, junctional complexes, and basal lamina. In marked contrast, tumor cells covered explant surfaces forming an irregular, disorganized layer of squamous-like cells with elongated nuclei and prominent nucleoli. Microvilli, junctional complexes, and basal lamina were poorly developed or absent. Intercellular attachments appeared tenous. Some tumor cells accumulated lipid; synthesis of mucus-like material was not seen. At later intervals of culture up to 10 weeks, synthesis of mucus-like material by basal cells, or their derivatives, declined. Surface cells of neoplastic prostate gradually became more anaplastic in appearance; cells contacted neighboring cells with pseudopodia and filopodia.
通过光镜（LM）、透射电镜（TEM）和扫描电镜（SEM）在连续间隔时间监测人前列腺肿瘤对长期外植体培养的形态学反应，并与正常前列腺进行比较。外植体在补充有胎牛血清和抗生素的CMRL - 1066中于37℃培养。在培养0时，从刚尸检获得的年轻成年男性正常前列腺由腺腔和导管组成，内衬柱状至立方分泌上皮细胞以及嵌入纤维肌肉基质中的基底细胞。肿瘤组织通过经尿道切除术（TUR）手术获得，由广泛浸润的基质组成，其中排列着大小不一的腺样结构的高分化至中分化肿瘤细胞。分泌活性明显；这些腺体中不存在基底细胞。在培养的早期直至数周，正常前列腺的分泌细胞发生坏死。基底细胞保持存活，重新填充腺泡结构并使外植体表面上皮化。在这些部位，基底细胞或其衍生物形成多细胞上皮。细胞间间隙增大，可见黏液样物质的合成。上皮特征包括微绒毛、连接复合体和基膜。形成鲜明对比的是，肿瘤细胞覆盖外植体表面，形成一层不规则、无序的鳞状样细胞层，细胞核细长，核仁突出。微绒毛、连接复合体和基膜发育不良或不存在。细胞间连接似乎很脆弱。一些肿瘤细胞积累脂质；未见黏液样物质的合成。在培养后期直至10周，基底细胞或其衍生物的黏液样物质合成减少。前列腺肿瘤的表面细胞外观逐渐变得更加间变；细胞通过伪足和丝状伪足与相邻细胞接触。
