Schnaitman C A
Proc Natl Acad Sci U S A. 1969 Jun;63(2):412-9. doi: 10.1073/pnas.63.2.412.
An improved method is described for dissolving membrane proteins and for resolving them by polyacrylamide gel electrophoresis. With this procedure, the inner mitochondrial membrane has been found to contain 23 different protein species and the outer membrane 12. Only one protein species appears to be common to the two membranes. Smooth and rough microsomal membranes contain 15 different proteins. The approximate molecular weight and relative amount of each protein species have been extrapolated from the gel patterns. The outer mitochondrial membrane and the smooth microsomal membrane contain at least three protein species that appear to be identical, suggesting that they possess a common origin.
本文描述了一种用于溶解膜蛋白并通过聚丙烯酰胺凝胶电泳对其进行分离的改进方法。通过该方法,发现线粒体内膜含有23种不同的蛋白质,外膜含有12种。似乎只有一种蛋白质是这两层膜共有的。光滑型和粗糙型微粒体膜含有15种不同的蛋白质。已从凝胶图谱推断出每种蛋白质的近似分子量和相对含量。线粒体外膜和光滑型微粒体膜至少含有三种似乎相同的蛋白质,这表明它们有共同的起源。